Fig. 4: SNP mediated differential expression in the transgenic proestrus uterus. | Nature Communications

Fig. 4: SNP mediated differential expression in the transgenic proestrus uterus.

From: A common allele increases endometrial Wnt4 expression, with antagonistic implications for pregnancy, reproductive cancers, and endometriosis

Fig. 4

A Effect of the SNP on flanking genes, with differences in Wnt4 expression between the wildtype and transgenic lines, but not Cdc42, was confirmed in the RNAseq analysis. B Volcano plot showing significance and effect size distribution. Log2 fold change values are calculated with respect to TG such that downregulated genes have a negative value and upregulated genes have a positive value. Significantly differentially expressed genes (adj. P > 0.05) are in red. Genes associated with proliferation are labeled in black and Wnt4 is indicated in red. C Heatmap of significantly differentially expressed genes with an absolute log2 fold change > 0.5. Genes with functions of relevance to reproductive phenotypes were among those with the greatest increase in expression. All plots are based on independent transcriptomes of 4 TG and 3 WT individuals Boxplots show the median (central line) and interquartile range (50% of the data fall within the box), with the whiskers covering the range of data (Q1 or Q3 + 1.5IQR).

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