Fig. 4: Enriched metabolic pathways within the gut microbiome profile.

a PICRUSt2 analysis showing enrichment of metabolic pathways at C3d4 and C6d4. The dot plot summarizes all enriched pathways (p < 0.05) as a function of group (denoted by color, n = 12 per group) and the combined enrichment score (calculated as –log (p) x delta). The right panel highlights pathways relevant to the observed metabolome changes (Fig. 2). For statistical details please see Supplementary Data 2. b PICRUSt2 analysis for C6d8 separated for sex and presented as a horizontal bar graph separated for AL (dark gray) and PRF (red).