Fig. 5: Disease history affects CDV-induced TFF upregulation. | Nature Communications

Fig. 5: Disease history affects CDV-induced TFF upregulation.

From: Therapeutic mitigation of measles-like immune amnesia and exacerbated disease after prior respiratory virus infections in ferrets

Fig. 5: Disease history affects CDV-induced TFF upregulation.The alternative text for this image may have been generated using AI.

a Schematic of study design. b, c PBMC-associated CDV viremia (b) and nasal lavage (d) titers of ferrets infected with recCDV-5804p NΔ425-479. d Survival of consecutively IAV + CDV-infected ferrets after treatment of primary IAV with 4-FlU. e–g PBMC-associated primary viremia titers (e), circulating lymphocyte counts (f), and survival (g) of ferrets infected with recCDV-5804p NΔ425-479 67 dpi with primary pdmCA09. Symbols represent geometric means ± geometric SD (b, c, e, f), lines intersect means; log-rank (Mantel-Cox) test, median survival is stated (d, g); n numbers as specified. h RNAseq screen of differentially expressed transcripts present in BAL fluids extracted 5 dpi with CDV from consecutively infected versus CDV-only ferrets. Differentially expressed genes were determined with the Wald test and P values corrected for multiple tests using the method of Benjamini and Hochberg. Significance thresholds (adjusted p < 0.01, log2 fold change > 2) are shown as dotted horizontal and vertical lines, respectively. i Trefoil factor (TFF1, TFF2, and TFF3) transcripts in BAL fluids harvested 5 dpi with CDV from consecutively infected versus CDV-only ferrets. j RT-qPCR quantitation of relative presence of trefoil factor-encoding message in lung tissue of consecutively infected versus CDV-only ferrets, extracted 10 dpi with CDV. Symbols (i, j) represent individual animals, bars show range; 1-way ANOVA with Dunnett’s post-hoc test (i, j); n = 3. Source data are provided as a Source Data file.

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