Table 3 Kinetic parameters of ALDH mutants

From: RETRACTED ARTICLE: Systems engineering of Escherichia coli for high-level glutarate production from glucose

ALDH

K M(mM)

k cat(min-1)

k cat/K M(min-1·mM-1)

Fold increase in k cat/K M

WT

12.2 ± 0.1

6.8 ± 0.3

0.6

1.0

Mu1

10.3 ± 0.5

16.3 ± 0.2

1.6

2.7

Mu2

14.5 ± 0.3

19.5 ± 0.1

1.34

2.2

Mu3

3.5 ± 0.2

15.6 ± 0.5

4.5

7.5

Mu4

4.9 ± 0.5

146.1 ± 0.5

29.8

49.6

Mu5

6.2 ± 0.3

189.5 ± 0.5

30.6

51.0

  1. Replications size n = 3. All data are reported as mean ± standard deviation.
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