Fig. 1: Schematic drawings of the BSNFs-chip design and the sample preparation process. | Nature Communications

Fig. 1: Schematic drawings of the BSNFs-chip design and the sample preparation process.

From: Biporous silica nanostructure-induced nanovortex in microfluidics for nucleic acid enrichment, isolation, and PCR-free detection

Fig. 1: Schematic drawings of the BSNFs-chip design and the sample preparation process.

The polyethylene terephthalate (PET) film serves as a substrate for nanofilm synthesis and the construction of a sample preparation chip. The biporous silica nanofilm (BSNF) is formed by synthesizing a small-pore porous silica nanofilm (PSNF) on the PET film, followed by sequential synthesis of a large-pore silica nanofilm on the PSNF. The BSNFs-chip is fabricated by assembling two PET films, which have been synthesized with the BSNF, into a microchannel-engraved tape. The BSNF surfaces corresponding to the microchannel are modified with amine-functional groups for sample binding. When samples and ADH are injected into the BSNFs-chip, the amine-functional groups of the BSNF surface bind ADH and pathogens, resulting in pathogen enrichment. This enrichment process is more efficient due to the high surface area and nanovortex of the BSNF. A lysis buffer is added to the microchannel to extract nucleic acids from the enriched pathogens, and the enriched nucleic acid sample is collected using an elution buffer.

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