Fig. 5: Linoleic acid (LA) inhibited Th17 and promoted Treg cell differentiation in vitro. | Nature Communications

Fig. 5: Linoleic acid (LA) inhibited Th17 and promoted Treg cell differentiation in vitro.

From: Porphyromonas gingivalis aggravates colitis via a gut microbiota-linoleic acid metabolism-Th17/Treg cell balance axis

Fig. 5: Linoleic acid (LA) inhibited Th17 and promoted Treg cell differentiation in vitro.

a MACS-sorted naïve CD4+ T cells were cultured with anti-CD3/CD28 beads for three days. The expression of IL-17 and Foxp3 in naïve CD4+ T cells cocultured with different concentrations of LA detected by flow cytometry. b The expression of IL-17 and Foxp3 in naïve CD4+ T cell culture conditions induced with TGF-β under different concentrations of LA was detected by flow cytometry. c The expression of IL-17 and Foxp3 in naïve CD4+ T cell culture conditions induced with TGF-β and IL-6 under different concentrations of LA was detected by flow cytometry. d, f, h The proportion of CD4+IL-17+ Th17 cells to total CD4+ T cells in each group was statistically analysed. n = 4 biologically independent samples. e, g, i The proportion of CD4+Foxp3+ Treg cells to total CD4+ T cells in each group was statistically analysed. n = 4 biologically independent samples. Data are presented as the mean ± SEM; ns: no significant difference; *p < 0.05; **p < 0.01; ***p < 0.001 by two-tailed one-way ANOVA. Source data are provided as a Source Data file.

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