Fig. 2: DDX39A and DDX39B contain generic A-body targeting motifs.
From: Protein thermal sensing regulates physiological amyloid aggregation
a DDX39A (left panel) and DDX39B (right panel) N-terminal truncation mutations were expressed in heat shock-treated MCF-7 cells. A-body targeting efficiency was calculated for each protein fragment. 10 cells were analyzed per replicate, and values represent means ± s.e.m (n  =  3 independent experiments, a two-tailed unpaired Student’s t test was used: *p ≤ 0.05). b Cartoon 3D structure of DDX39B (PDB: 1XTI) with highlighted minimal A-body targeting motifs: amino acids 100−150 (yellow), amino acids 150−200 (pink), and amino acids 200−250 (cyan). c A Thioflavin T assay was performed with DDX39A (1−39), DDX39A (160−199), DDX39B (1−40), and DDX39B (161−200) peptides. Endpoint (16 h) Thioflavin T fluorescence is presented in arbitrary units (A.U.) as means ± s.e.m. (n = 3 independent experiments, a two-tailed Student’s t test was used: *p ≤ 0.05). d IUPred3 disorder prediction maps of DDX39A and DDX39B. A schematic of the generic DDX39 protein and the putative domains is included (above). Source data for all graphs are provided with this paper.
