Fig. 6: CD48 mediates ADCC sensitivity through NK activity regulation.

a Representative flow cytometry analysis of CD48 expression in H929 cells transfected with indicated sgRNAs. b Normalized lysis percentage of CD48 WT or KO H929 cells after co-culture with primary NK cells at different E:T ratios with IL-2 for 6 hours (mean ± SEM, n = 3 biologically independent experiments). ***p < 0.001 (two-sided student’s t test). c CD48 WT and KO H929 cells were co-cultured with primary human NK cells and Dara at different E:T ratios, and subjected to ADCC assay (mean ± SEM, n = 3 biologically independent experiments). **p < 0.01; ***p < 0.001 (two-sided student’s t test). d Representative flow cytometry analysis of CD48 expression in KDM6A KO and control H929 cells after ectopic overexpression of CD48. e ADCC assay of KDM6A KO and WT H929 cells after ectopic overexpression of CD48 and co-cultured with primary human NK cells and Dara at different E:T ratios (mean ± SEM, n = 3 biologically independent experiments). ***p < 0.001 (two-sided student’s t test). f Intracellular IFN-γ staining of primary NK cells co-cultured with KDM6A WT or KO H929 cells after ectopic overexpression of CD48 with Dara for 6 hours (mean ± SEM, n = 3 biologically independent experiments). ns, not significant; **p < 0.01 (two-sided student’s t test). KDM6A KO or control H929 cells after ectopic overexpression of CD48 were co-cultured with Dara and primary NK cells for 6 hours, and the supernatant was collected for granzyme B (g) and perforin (h) ELISA assay (mean ± SEM, n = 3 biologically independent experiments). ns, not significant; **p < 0.01 (two-sided student’s t test). Source data are provided as a Source Data file.