Fig. 4: Intestinal IL-22RA1-mediated alterations in microbiota composition regulate systemic metabolism and inflammation. | Nature Communications

Fig. 4: Intestinal IL-22RA1-mediated alterations in microbiota composition regulate systemic metabolism and inflammation.

From: Intestinal IL-22RA1 signaling regulates intrinsic and systemic lipid and glucose metabolism to alleviate obesity-associated disorders

Fig. 4

A Genus-level heatmap based on Euclidian distance with average linkage using Hierarchical clustering of 16S rRNA sequencing data derived from fecal DNA of Il22ra1fl/fl;Villin-cre mice before (day 0) and after (day 105) HFD. B Volcano plot displaying a differential expression of bacterial genera from Il22ra1fl/fl;Villin-cre mice after HFD. C RT-PCR analysis of epididymal WAT Mmp12 expression from Il22ra1fl/fl;Villin-cre mice fed a control diet or HFD for 16 weeks. D Representative immunofluorescence image of MMP12 staining of epididymal WAT from HFD-fed Il22ra1fl/fl;Villin-cre mice. E Weight gain of HFD-fed Il22ra1fl/fl;Villin-cre mice after 4 weeks of antibiotics treatment starting at week 12 (indicated by dotted line). F GTT from HFD-fed Il22ra1fl/fl;Villin-cre mice after antibiotics treatment. G Representative H&E images of liver tissue from antibiotics treated and HFD-fed Il22ra1fl/fl;Villin-cre mice. H RT-PCR analysis of glucose (Foxo1, G6pc) and lipid (Acc, Ppara) metabolism genes from the liver of antibiotics treated and HFD-fed Il22ra1fl/fl;Villin-cre mice. I Representative H&E images of epididymal WAT from antibiotics treated and HFD-fed Il22ra1fl/fl;Villin-cre mice. J RT-PCR analysis of lipid metabolism genes from the epidydimal WAT of antibiotics-treated and HFD-fed Il22ra1fl/fl;Villin-cre mice. Figures 4A–B, 4E–J are representative of 2 independent experiments. Figure 4A, B are representative of N = 6 cre- and 5 cre+ mice. Figure 4C is representative of N = 4 (control, cre-), 4 (control, cre+), 14 (HFD, cre-), and 17 (HFD, cre+) mice from 2 (control) or 9 (HFD) independent experiments. Figure 4D is representative of N = 4 cre- and 3 cre+ control diet-fed mice, N = 4 cre- and 5 cre+ HFD-fed mice, and 2 (control diet) and 3 (HFD) independent experiments. Figure 4E–J are representative of N = 4 cre- and 4 cre+ mice. Data are presented as mean ± SEM in all graphs. 2-way ANOVA with Sidak multiple comparisons in 4C, 4E, F; Unpaired t-test, one-tailed in B; Mann–Whitney test, two-tailed in H, J. Scale bar = 50 μm.

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