Fig. 7: Paneth cell-specific IL-22RA1 signaling mediates their antimicrobial functions and systemic fasting glucose metabolism after HFD. | Nature Communications

Fig. 7: Paneth cell-specific IL-22RA1 signaling mediates their antimicrobial functions and systemic fasting glucose metabolism after HFD.

From: Intestinal IL-22RA1 signaling regulates intrinsic and systemic lipid and glucose metabolism to alleviate obesity-associated disorders

Fig. 7: Paneth cell-specific IL-22RA1 signaling mediates their antimicrobial functions and systemic fasting glucose metabolism after HFD.The alternative text for this image may have been generated using AI.

A RT-PCR analysis of Paneth cell-associated antimicrobial genes from ileal tissues of Il22ra1fl/fl;Villin-cre mice. B Representative immunofluorescence images of LYZ1 staining from Il22ra1fl/fl;Villin-cre mice. C Average number of LYZ1+ cells/crypt for each mouse from Fig. 7B. D RT-PCR analysis of Paneth cell-associated genes from sorted Lgr5-GFPhigh (ISC) and Lgr5-GFPlow (progenitor) cells from Lgr5 reporter mice. E RT-PCR analysis of organoids cultured with pure media, rIL-22, palmitic acid (PA), or PA and rIL-22. Data depict the fold change of the treated groups. F GTTs from chow diet-fed Defa6-cre-/+, ROSA26DTA mice. G Representative immunofluorescence images of LYZ1 staining from Il22ra1fl/fl;Defa6-cre mice. H Average number of LYZ1+ cells/crypt from Fig. 7G. I Weight gain of HFD-fed Il22ra1fl/fl;Defa6-cre mice. J Glucose values from Il22ra1fl/fl;Defa6-cre mice after fasting. Figure 7A represents N = 5–6 (chow diet, cre-), 5–6 (chow diet, cre+), 4 (HFD, cre-), and 8 (HFD, cre+) mice per group and 3 independent experiments. Figure 7B, C represent N = 4 (chow diet, cre-), 4 (chow diet, cre+), 4 (HFD, cre-), and 5 (HFD, cre+) mice from 2 independent experiments. Figure 7D represents N = 3 control diet-fed mice and 4 HFD-fed mice from 2 independent experiments. Figure 7E represents N = 5 mice from 2 independent experiments. Figure 7F represents N = 3 cre- and 4 cre+ mice from 2 independent experiments. Figure 7G, H represent N = 4 (chow diet, cre-), 4 (chow diet, cre+), 5 (HFD, cre-), and 5 (HFD, cre+) mice from 1 (chow) or 3 (HFD) independent experiments. Figure 7I represents N = 5 cre- and 7 cre+ mice from 4 independent experiments. Figure 7J represents N = 11 cre- and cre+ mice from 4 independent experiments. Data are presented as mean ± SEM in all graphs. Mann–Whitney test, two-tailed in 7J; 1-way ANOVA with Tukey multiple comparisons in 7E; 2-way ANOVA with Sidak multiple comparisons in 7A, 7C, 7D, 7F, 7H, 7I. Scale bar = 20 μm.

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