Fig. 5: Label-free hyperspectral detection of different lipid subtypes in situ using PRM-SRS. | Nature Communications

Fig. 5: Label-free hyperspectral detection of different lipid subtypes in situ using PRM-SRS.

From: Multi-molecular hyperspectral PRM-SRS microscopy

Fig. 5

a, b SRS image of a human kidney (n = 1, no replicate trials) tissue section at 2850 cm−1. Panel b shows the enlarged image of the boxed area in panel (a). Hollow arrowheads, intracellular lipid droplets in tubules. Solid arrowheads, eosinophilic bodies. Circles, lipid droplets sequestered by podocytes in the glomerulus. Scale bar, 200 μm. c PRM-SRS spectra and images of different lipid subtypes of interest show the distribution of the similarity scores, each with the same contrast levels. Spectra of the top 10% of similarity score pixels overlaid on the reference spectrum for each lipid subtype show consistent matches. Resulting similarity score images were background subtracted to improve the contrast. Center-line of spectra correspond to mean spectrum for each respective lipid subtype with 1 standard deviation (SD) error bands. Scale bar, 200 µm. TAG triacylglycerides, Cer ceramide. Source data are provided as a Source Data file.

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