Fig. 5: Comparison of Dolphyn and Pepsyn results.

A A significant difference in phage reactivity can be observed in mean PhARscore of Pepsyn and Dolphyn designed libraries, when splitting the 112 (Pepsyn) and 88 (Dolphyn) phages into two groups according to their taxonomic annotation (2-sided Wilcoxon test). The horizontal lines in the boxplots indicate the median, and the lower and upper value of the box the interquartile ranges (25 and 75 percentiles). The whiskers extend to the most extreme data point which is no more than 1.5 times the interquartile range from the box. B The phage reactivities detected in more samples in the Gut Phage Database (x-axis) were also reactive in more individuals in our cohort (y-axis). Phages were only considered if present in both library designs. The spearman correlation is calculated based on reactivity and p-values reported based on a 2-sided test. C Phage proteins from phages with PhARscores indicating high reactivity are displayed in sequential order (no shared proteins) for 5 individuals. Note that the Dolphyn library contains less peptides per protein and has consequently a lower number of reactive peptides per protein. D Protein discovery power. Using reactivities to Pepsyn proteins as ground truth, performance metrics are shown for both Pepsyn and Dolphyn peptides in recovering protein by sample reactivities. E Principal component analysis clustering of phages colored by different annotations, with the first principal component (PC) on the x-axis and the second PC on the y-axis. The PCs of phages according to the sample-reactivity vectors per phage are similar for both libraries. Phages with same annotations and clustering in the heatmap (Fig. 4) group together independently of the library design. | Source data are provided as a Source Data file.