Fig. 1: Structure of the E2 enzyme UBE2E1 in complex with a SETDB1-derived peptide.
From: Structure-guided engineering enables E3 ligase-free and versatile protein ubiquitination via UBE2E1
a In vitro UBE2E1-dependent ubiquitination assay using hexapeptide (KEGYES)-fused EGFP as substrates. Gel images are representative of independent biological replicates (n = 2). b Mass spectrometry confirms the ubiquitination product (EGFP*-Ub) and the lysine on the hexapeptide is the ubiquitination site. The observed (obs.) and calculated (calc.) molecular weights were marked. c Overall structure of UBE2E1 bound to hexapeptide. UBE2E1 is shown as ribbon and colored in bright blue. The peptide is shown as sticks: the long side, corner, and the short side of the “L shape”-hexapeptide are colored in bright orange, green and cyan, respectively. Source data are provided as a Source Data file.
