Fig. 2: clueless regulates sisR-1 expression.
From: Nutrient-dependent regulation of a stable intron modulates germline mitochondrial quality control
A Relative expression of sisR-1 in Oregon R and clu[d08713] heterozygous stage 14 oocytes. Data are presented as mean values +/−SD from three biological replicates. *p < 0.05. Two-tailed t-test. B Confocal images of stage 8/9 egg chambers showing localization of sisR-1 (Green), ATP5α (Red) and DAPI (Blue) in fed Oregon R and clu[d08713] heterozygous. Arrowheads point to colocalization of sisR-1 and mitochondrial clumps. Scale bar = 10 µm. C Confocal images of stage 8/9 egg chambers showing localization of ATP5α in clu[d08713]/+, clu[d08713]/+;sisR-1 RNAi line 1 and 2. Arrowheads point to mitochondrial clumps. Scale bar = 20 µm. D Percentages of stage 8/9 egg chambers with mitochondrial clumps in clu[d08713]/+, clu[d08713]/+;sisR-1 RNAi line 1 and 2 (n = 100 egg chambers) *p < 0.001. Chi-square test, one-sided. E Number of mitochondrial clumps present in stage 8/9 egg chambers that contain clumps in clu[d08713]/+, clu[d08713]/+;sisR-1 RNAi line 1 and 2. Data are presented as mean values +/−SD. **p < 0.05. n = 54 (clu/+), 25 (clu/+;RNAi #1), 18 (clu/+;RNAi #2) egg chambers. Two-tailed t-test. F Enrichment of sisR-1, but not U85, in Clu-GFP immunoprecipitate compared to IgG control. G Relative levels of sisR-1 normalized to Rp49 after alpha-amanitin treatment in Oregon R and clu ovaries. Data are presented as mean values +/−SD from three biological replicates. *p < 0.05. Two-tailed t-test. H Model on the effects of fasting on Clu protein and sisR-1 in promoting mitochondrial clump formation. Source data are provided as a Source Data file.
