Fig. 3: DIP1 regulates sisR-1 expression during fasting.

A Relative expression of DIP1 normalized to actin5C in fed and fasted Oregon R stage 14 oocytes. Error bars depict SD from two biological replicates. ns: not significant, p > 0.05. Two-tailed t-test. B Levels of DIP1 protein in ovaries of fed and fasted Oregon R flies. Graph shows the quantified relative levels of DIP1 normalized to Actin. *p < 0.05. Data are presented as mean values +/−SD from three biological replicates. Two-tailed t-test. C Confocal images of stage 8/9 egg chambers showing localization of DIP1 (Green) and DAPI (Blue) in fed and fasted Oregon R. Scale bar = 20 µm. D Confocal images of stage 8/9 egg chambers showing localization of sisR-1 (Green), ATP5α (Red) and DAPI (Blue) in fed Oregon R, DIP1−/−, clu−/− and DIP1−/−;clu−/−. Scale bar = 10 µm. E Percentages of nurse cell per stage 8/9 egg chamber with mitochondrial clumps in fed Oregon R, DIP1−/−, clu−/− and DIP1−/−;clu −/−. Data are presented as mean values +/−SD. ***p < 0.001. n = 9 (Oregon R), 7 (DIP1−/−), 4 (clu−/−) and 9 (DIP1−/−;clu −/−) egg chambers counted. Two-tailed t-test. F Relative expression of sisR-1 by smFISH in the nucleus and cytoplasm of stage 8/9 egg chambers in fed Oregon R, DIP1 −/−, clu−/− and DIP1−/−;clu −/−. Data are presented as mean values +/−SD. ***p < 0.001. n = 9 (N), 10 (C) for Oregon R, 10 (N) and 13 (C) for DIP1−/−, 8 (N) and 10 (C) for clu−/− and 6 (N) and 5 (C) for DIP1−/−;clu−/−. egg chambers. Two-tailed t-test. G Relative levels of sisR-1 in fed Oregon R, DIP1−/−, clu−/− and DIP1−/−;clu−/− oocytes. Data are presented as mean values +/−SD from three biological replicates. *p < 0.05 **p < 0.01. Two-tailed t-test. H Images of dissected ovary pairs of fed Oregon R, DIP1−/−, clu−/− and DIP1−/−;clu−/− taken at 1.5X zoom. I Proposed model on the effects of fasting on DIP1, Clu and sisR-1 in promoting mitochondrial clump formation. Source data are provided as a Source Data file.