Fig. 5: Clearance of fasting-induced mitochondrial clumps improves oocyte quality.

A Stage 8/9 egg chambers showing fasting-induced mitochondrial clumping persists in Oregon R but not in sisR-1 RNAi ovaries. B Hatch rates 2 days after egg laying in fasted Oregon R and sisR-1 RNAi. *p < 0.05. Chi-square test, one-sided. n = 183 eggs in 20 experiments (Oregon R) and 86 eggs in 7 experiments (RNAi). C Stage 8/9 egg chambers showing sisR-1 expression in initially fed and fasted Oregon R flies and after being subsequently fed. D Hatch rates of Oregon R females that underwent the indicated duration of feeding and fasting regimes. Data are presented as mean values +/−SD. *p < 0.05. ns not significant. Two-tailed t-test. n = 3 (16 h fed), 3 (16 h fasted), 6 (30 h fed), 8 (30 h fasted) biological replicates. E Confocal images of ATP5α localization in fed or fasted Oregon R ovaries at 16 or 30 h. Arrowheads point to mitochondrial clumps. Scale bar = 20 µm. F Percentages of nurse cell per stage 8/9 egg chamber with mitochondrial clumps in fed or fasted Oregon R ovaries at 16 or 30 h. Data are presented as mean values +/−SD. **p < 0.01, ***p < 0.001. n = 7 (16 h fed), 7 (16 h fasted), 3 (30 h fed), 6 (30 h fasted) egg chambers counted. Two-tailed t-test. G Confocal images of sisR-1 (Green) and DAPI (Blue) localizations in fed or fasted Oregon R ovaries at 16 or 30 h. Arrowheads point to mitochondrial clumps. Scale bar = 20 µm. H Levels of sisR-1 normalized to actin5C in fed or fasted Oregon R ovaries at 16 or 30 h. Data are presented as mean values +/−SD. n = 4 biological replicates. Two-tailed t-test. I Relative expression of sisR-1 by smFISH in the cytoplasm of stage 8/9 egg chambers in fed or fasted Oregon R ovaries at 16 or 30 h. Data are presented as mean values +/−SD. ***p < 0.001. n = 6 (16 h fed), 6 (16 h fasted), 8 (30 h fed), 9 (30 h fasted) egg chambers counted. Two-tailed t-test. Source data are provided as a Source Data file.