Fig. 7: Summary of the mechanism of trabectedin-induced TC-NER-mediated SSB formation and toxicity, and the development of TRABI-Seq. | Nature Communications

Fig. 7: Summary of the mechanism of trabectedin-induced TC-NER-mediated SSB formation and toxicity, and the development of TRABI-Seq.

From: Trabectedin derails transcription-coupled nucleotide excision repair to induce DNA breaks in highly transcribed genes

Fig. 7

Trabectedin-DNA adducts are exclusively recognized by TC-NER, and not by GG-NER. The adducts then block the incision of the XPG endonuclease, causing persistent XPF-mediated breaks. Mapping those breaks in a genome-wide fashion displays where TC-NER is active: predominantly in highly transcribed genes and their upstream regions due to a link to divergent transcription.

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