Fig. 2: 5-HTergic stimulation induces structural long-term potentiation of individual dendritic spines on PFC layer 2/3, but not layer 5, pyramidal neurons.
From: Serotonin modulates excitatory synapse maturation in the developing prefrontal cortex
a Schematic of experimental timeline. b Time-lapse 2 P images of a dendrite after 5-HT HFU (30 pulses of 1 ms duration at 1 Hz by 810 nm) and a control dendrite from the same neuron. Purple cross represents 5-HT HFU point; purple arrow indicates the target spine 35 min post 5-HT HFU. c Time course of spine size changes following 5-HT HFU (purple indicator shows 5-HT HFU time point) and (d) summary of spine size changes at 35 min post 5-HT HFU. [HFU EP11-14: n = 30 spines, 16 cells; Off-target: 13 ROIs, 13 cells; Shift HFU: 22 spines, 8 cells; HFU EP15-20: 22 spines, 12 cells; two-tailed paired t-test (HFU EP11-14, 5 min: p = 0.0236, 15 min: p = 0.0006, 25 min: p = 0.0036, 35 min: p = 0.0015; Off-target EP11-14, 35 min: p = 0.3295; HFU EP15-20, 35 min: p = 0.219); two-tailed Mann-Whitney (Off-target: p = 0.0135; Shift HFU: p = 0.004; HFU EP15-20: p = 0.0257)]. e Summary of spine size changes at 35 min post 2 P 5-HT uncaging [LFU target: n = 23 spines, 15 cells; HFU target Layer 5: 28 spines, 14 cells; two-tailed Mann-Whitney (LFU target: p = 0.0332; HFU target Layer 5: p = 0.0264); 5-HT LFU: 30 pulses of 1 ms duration at 0.1 Hz by 810 nm]. HFU EP11-14 data (filled purple bar) are from Fig. 2d. *p < 0.05, **p < 0.01; error bars represent SEM. n.s., not significant. Source data are provided as a Source Data file.
