Fig. 3: Mislocalization of mutant rhodopsin expressed in HEK293 cells that were untreated or treated with 15 μM 9-cis retinal.
From: Aggregation of rhodopsin mutants in mouse models of autosomal dominant retinitis pigmentosa
A Singly expressed rhodopsin. Each row shows images of fluorescence from YFP-tagged WT, P23H, or G188R rhodopsin (green), the ER marker DsRed2-ER (red), the plasma membrane (PM) marker WGA (magenta), overlays of the fluorescence from YFP-tagged rhodopsin and the ER marker (yellow), and overlays of the fluorescence from YFP-tagged rhodopsin and the PM marker (white). B HEK293 cells coexpressing mTq2-tagged WT rhodopsin and either YFP-tagged P23H or G188R rhodopsin. Each column shows images of fluorescence from YFP-tagged mutant rhodopsin (red), mTq2-tagged WT rhodopsin (green), overlays of the fluorescence from YFP-tagged mutant rhodopsin and mTq2-tagged WT rhodopsin (yellow), PM marker (blue), overlays of fluorescence from YFP-tagged mutant rhodopsin and the PM marker (magenta), and overlays of fluorescence from mTq2-tagged WT rhodopsin and the PM marker (cyan). Nuclei were stained by DAPI (blue) and were shown in all images except the last 3 columns in B. Scale bar, 5 μm. Images are representative of at least three different experiments.
