Fig. 3: YAP mediates FLNA downregulation in activated CTLs.

a The expression of YAP in human CD8⁺ Tn and Teff cells were analyzed by western blot. b Human CD8⁺ Teff cells were transfected with or without YAP siRNA, and FLNA expression was determined by western blot. c Human CD8⁺ Teff cells were treated with DMSO or verteporfin (VP) (1 μM) for 24 h, and FLNA expression was determined by western blot. d ChIP–qPCR analysis of YAP enrichment around the promoters of FLNA in human CD8⁺ Tn and Teff cells. e 293 T cells were co-transfected with FLNA promoter-luciferase reporter PGL4 and YAP plasmid for 24 h, followed by analysis of luciferase activity. f The stiffness of human CD8⁺ Teff cells transfected with or without YAP siRNA was determined by AFM. g The stiffness of human CD8⁺ Teff cells treated with DMSO or verteporfin (1 μM) for 24 h was determined by AFM. h Human CD8⁺ Teff cells transfected with or without YAP siRNA were stained with DAPI (blue), phalloidin (F-actin, green); Scale bar, 10 μm. i Human CD8⁺ Teff cells treated with DMSO or verteporfin (1 μM) for 24 h were stained with DAPI (blue), phalloidin (F-actin, green); Scale bar, 10 μm. j The expression of TEAD1 in human CD8⁺ Tn and Teff cells were analyzed by western blot. k The expression of FLNA in human CD8⁺ Tn cells or Teff cells transfected with or without TEAD1 siRNA was determined by western blot. l The stiffness of human CD8⁺ Teff cells transfected with or without TEAD1 siRNA was determined by AFM. m ChIP–qPCR analysis of TEAD1 enrichment around the promoters of FLNA in human CD8⁺ Tn and Teff cells. n 293 T cells were co-transfected with WT or mutant FLNA promoter-luciferase reporter PGL4 and TEAD1 plasmid for 24 h, followed by analysis of luciferase activity. n = 3 independent experiments (a–e, j, k, m and n); n = 10 independent experiments (f–i and l). The data are represented as mean ± SD. p value by One-way ANOVA Bonferroni’s test (d–f, h and l–n); p value by two-tailed Student’s t-test (g and i). Source data are provided as a Source Data file.