Fig. 6: T-leukemic cells usurp the softness to evade CTL killing in vivo.

a Schematic of the mouse model is shown, NSG mice were transplanted with 2 × 10⁵ OVA-expressing ICN1 leukemic cells. At 7 days post engraftment, NSG mice were treated or untreated with Jas (50 μg/kg), followed by adoptively transferring with or without 5 × 10⁶ OT-I T cells. At 10 days post engraftment, PI⁺ cells in the ICN1 cells were analyzed by flow cytometry. At 15 days post engraftment, ICN1 leukemic cells dissemination were determined by flow cytometry (Figure was created with BioRender.com). b PI⁺ cells from spleen and bone marrow were analyzed by flow cytometry. Data from six individual mice were plotted and shown on the right. c The stiffness of ICN treated with Jas in vivo was determined by AFM; n = 10 independent experiments. d Kaplan–Meier survival curves of OVA-expressing ICN1 leukemic cells in each group as indicated. ICN1 GFP⁺ cells from spleen and bone marrow were analyzed by flow cytometry (e); Data from six individual mice were plotted and shown on the right (f). ICN1, intracellular Notch 1. The data are represented as mean ± SD. p value by One-way ANOVA Bonferroni’s test (b and f); p value by two-tailed Student’s t-test (c); P value by Log-rank survival analysis (d). Source data are provided as a Source Data file.