Fig. 9: Softness promotes T-leukemic cell immune evasion in patients.

a The expression of FLNA, YAP and Y357-YAP in human normal T cells or primary T leukemia cells were analyzed by western blot. b The correlation between the expression of Y357-YAP and FLNA was showed. c The correlation between the expression of FLNA and cell stiffness was showed. d The correlation between the expression of Y357-YAP and cell stiffness was showed. e The cell viability of the primary T leukemia cells, primary B-leukemic and primary acute myeloid leukemic cells was detected by flow cytometry after coculturing with allogeneic donor-derived T cells for 12 h. f The cell viability of SGCTRL or FLNA-SGs T-leukemic cells was detected by flow cytometry after coculturing with allogeneic donor-derived T cells for 12 h. g The cell viability of SGCTRL, YAP KO, YAP KO/YAP WT and YAP KO/YAP MUT of T-leukemic cells was detected by flow cytometry after coculturing with allogeneic donor-derived T cells for 12 h. h The cell viability of T-leukemic cells treated with 200 nM Jas or DMSO was detected by flow cytometry after coculturing with allogeneic donor-derived T cells for 12 h. i The cell viability of T-leukemic cells treated 0.1 μM VP or DMSO was detected by flow cytometry after coculturing with allogeneic donor-derived T cells for 12 h. j Schematic of the experiment to test whether Jas or VP inhibits leukemia development in NSG mice. NSG mice were irradiated (2 Gy), then adoptively transferred with 1 × 10⁶ primary leukemic T cells labeled with PKH26 and 1 × 10⁶ allogeneic T cells. At 1 day post engraftment, NSG mice were treated with Jas (50 μg/kg) or VP (20 mg/kg). At 3 days post engraftment, PKH26⁺ cells were analyzed by flow cytometry (Figure was created with BioRender.com). k, l Representative flow plots and quantification of percent of PKH26⁺ primary T leukemia cells in bone marrow from mice treated with Jas (k) or VP (l). n = 10 biologically independent samples (a); n = 6 independent samples (e–i); n = 9 independent samples (k and l). The data are represented as mean ± SD. p value by One-way ANOVA Bonferroni’s test (e–g and k–l); p value by two-tailed Student’s t-test (h and i). Source data are provided as a Source Data file.