Fig. 1: dRNA HybISS workflow, experimental design and computational analysis.

A Schematic of using dRNA HybISS to study AKI. Schematic was created with BioRender. B A Julia package, CellScopes.jl, was developed for spatial data processing, analysis and visualization. Image was created with BioRender. C Outline of the spatial analysis pipeline used in this study, created with BioRender. D–F CellScopes.jl allows for visualization of gene expression on cells as data points (D), segmented polygons (E), and cell-type annotations (F).