Fig. 2: Major cell types and their spatial organizations in the kidney as revealed by dRNA HybISS. | Nature Communications

Fig. 2: Major cell types and their spatial organizations in the kidney as revealed by dRNA HybISS.

From: High resolution spatial profiling of kidney injury and repair using RNA hybridization-based in situ sequencing

Fig. 2

A Spatial distributions of major cell types in the whole kidney and close-ups of cell-type organizations in glomerulus, cortex, outer medulla, inner medulla, papilla and renal artery. Podo podocytes, JGA juxtaglomerular apparatus cells, gEC glomerular endothelial cells, aEC arterial endothelial cells, Fib fibroblasts, HealthyPT healthy proximal tubular cells, InjPT injured proximal tubular cells, TAL thick ascending limb of Loop of Henle, DCT distal deconvoluted tubule, CD-PC collecting duct principal cells, CD-IC collecting duct intercalated cells, Immune immune cells, Uro urothelial cells. B Expression known marker genes to define the cell-type identity. C Spatial distance between the two EC subtypes and the podocyte. n = 2812 podocytes. The line inside the box of the box plot represents the median and the boxes indicate 25th/75th percentile. Solid lines represent the full range of the distribution. Mann–Whitney U test was performed to determine the significance of the difference. D Change of transcript and cell proportion from cortex to papilla. **, P < 0.001; n.s. not significant.

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