Fig. 1: Liquid–liquid phase separation (LLPS) of coilin. | Nature Communications

Fig. 1: Liquid–liquid phase separation (LLPS) of coilin.

From: Large dynamics of a phase separating arginine-glycine-rich domain revealed via nuclear and electron spins

Fig. 1

a Sequence-based prediction of the propensity of coilin for RNA-mediated granule formation and LLPS. The shaded area shows the RG-rich region (residues 389-424) with high LLPS propensity. b-d The coilin peptide (clp) encompassing residues 389-424 undergoes LLPS after addition of polyU RNA, as revealed by visible turbidity in (b) and presence of droplets in light (c) and fluorescence microscopy (d). The fluorescent nucleic acid-binding dye (SYBR Gold) was used for tracking polyU RNA. In (e), the arrows show a few droplet pairs staying close to each other for a long time without being fused. f Fluorescence microscopy images of polyU-induced clp droplets after 3 days, showing droplets in pearls-string-like arrangements without fusion. g The control sample with polyU without clp did not show any droplet formation.

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