Table 1 Rate constants for the redox reaction of PfGrx, roGFP2, and related compounds

GSH

roGFP2^WT(S₂)

roGFP2^WT(SSG)

Not detectable

8.0/25 °C

PfGrx^C88S(SH)₂*roGFP2(S₂)

GSH

PfGrx^C88S(SH)₂

roGFP2^WT(SSG)

ca. 10 M^–1 s^{–1 a}

8.0/25 °C

PfGrx^C88S(SH)₂*GSH

roGFP2^WT(S₂)

PfGrx^C88S(SH)₂

roGFP2^WT(SSG)

ca. 10³M^–1 s^{–1 a}

8.0/25 °C

PfGrx^C88S(SH)₂

roGFP2^WT(S₂) + GSH

PfGrx^C88S(SH)₂

roGFP2^WT(SSG)

7.3 × 10⁵M^–2 s^–1

8.0/25 °C

PfGrx^DM(SH) ^b

roGFP2^WT(S₂) + GSH

PfGrx^DM(SH)

roGFP2^WT(SSG)

1.3 × 10⁵M^–2 s^–1

8.0/25 °C

PfGrx^C88S(SH)₂

roGFP2^WT(S₂) + GSH

PfGrx^C88S(SH)₂

roGFP2^WT(SSG)

3.4 × 10³M^–1 s^{–1 c}

8.0/25 °C

PfGrx^DM(SH) ^b

roGFP2^WT(S₂) + GSH

PfGrx^DM(SH)

roGFP2^WT(SSG)

6.5 × 10²M^–1 s^{–1 c}

8.0/25 °C

PfGrx^K26A/DM(SH) ^b

roGFP2^WT(S₂) + GSH

PfGrx^K26A/DM(SH)

roGFP2^WT(SSG)

36 M^–1 s^{–1 c}

8.0/25 °C

PfGrx^D90A/DM(SH) ^b

roGFP2^WT(S₂) + GSH

PfGrx^D90A/DM(SH)

roGFP2^WT(SSG)

5.1 ×10²M^–1 s^{–1 c}

8.0/25 °C

PfGrx^DM(SH) ^b

GSSCys

PfGrx^DM(SSG)

Cys

1.3 × 10⁶M^–1 s^{–1 d}

8.0/25 °C

PfGrx^K26A/DM(SH) ^b

GSSCys

PfGrx^K28A/DM(SSG)

Cys

6.9 × 10⁴M^–1 s^{–1 d}

8.0/25 °C

PfGrx^D90A/DM(SH) ^b

GSSCys

PfGrx^D90A/DM(SSG)

Cys

1.0 × 10⁶M^–1 s^{–1 d}

8.0/25 °C

PfGrx^C88S(SH)₂

roGFP2^C151S(SSG)

PfGrx^C88S(SSG)

roGFP2^C151S(SH)

4.9 × 10⁵M^–1 s^–1

8.0/25 °C

PfGrx^DM(SH) ^b

roGFP2^C151S(SSG)

PfGrx^DM(SSG)

roGFP2^C151S(SH)

6.4 × 10⁴M^–1 s^–1

8.0/25 °C

PfGrx^C88S(SH)₂

roGFP2^C208S(SSG)

PfGrx^DM(SSG)

roGFP2^C208S(SH)

1.4 × 10⁶M^–1 s^–1

8.0/25 °C

PfGrx^DM(SH) ^b

roGFP2^C208S(SSG)

PfGrx^DM(SSG)

roGFP2^C208S(SH)

2.5 × 10⁵M^–1 s^–1

8.0/25 °C

PfGrx^DM(SH) ^b

roGFP2^WT(SSG)

PfGrx^DM(SSG)

roGFP2^WT(SH)₂

ca. 8 × 10⁴M^–1 s^{–1 a}

8.0/25 °C

rxYFP(SH)₂-ScGrx1^WT(SH)₂

GSSG

rxYFP-ScGrx1^WT(SSG)

GSH

4 × 10³M^–1 s^{–1 e}

7.0/30 °C

rxYFP(SH)₂-ScGrx1^CxxS(SH)

GSSG

rxYFP-ScGrx1^CxxS(SSG)

GSH

3 × 10⁴M^–1 s^{–1 e}

7.0/30 °C

roGFP2(SH)₂-PfGrx^WT(SH)₂

GSSG

roGFP2-PfGrx^WT(SSG)

GSH

4.3 × 10⁷M^–1 s^–1

8.0/25 °C

roGFP2(SH)₂-PfGrx^C88S(SH)₂

GSSG

roGFP2-PfGrx^C88S(SSG)

GSH

1.4 × 10⁷M^–1 s^–1

8.0/25 °C

roGFP2(SH)₂-PfGrx^DM(SH) ^b

GSSG

roGFP2-PfGrx^DM(SSG)

GSH

2.6 × 10⁶M^–1 s^–1

8.0/25 °C

roGFP2^WT(SH)₂-PfGrx^WT(SSG)

roGFP2(SSG)-PfGrx^WT(SH)₂

9.7 s^–1

8.0/25 °C

roGFP2^WT(SH)₂-PfGrx^C88S(SSG)

roGFP2(SSG)-PfGrx^C88S(SH)₂

11.6 s^–1

8.0/25 °C

roGFP2^WT(SH)₂-PfGrx^DM(SSG) ^b

roGFP2(SSG)-PfGrx^DM(SH)

11.5 s^–1

8.0/25 °C

GSSG

rxYFP(SH)₂

rxYFP(SSG)

GSH

1.2 M^–1 s^{–1 f}

7.0/30 °C

GSSG

roGFP2^WT(SH)₂

roGFP2^WT(SSG)

GSH

0.7 M^–1 s^–1

8.0/25 °C

PfGrx^C88S(S₂)

roGFP2^WT(SH)₂

PfGrx^C88S(SS)roGFP2^WT

1.5 × 10²M^–1 s^–1

8.0/25 °C

PfGrx^C88S(SSG)

roGFP2^WT(SH)₂

PfGrx^C88S(SH)₂

roGFP2^WT(SSG)

8.6 × 10³M^–1 s^–1

8.0/25 °C

PfGrx^DM(SSG) ^b

roGFP2^WT(SH)₂

PfGrx^DM(SH)

roGFP2^WT(SSG)

3.0 × 10⁴M^–1 s^–1

8.0/25 °C

PfGrx^C88S(SSG)

roGFP2^C151S(SH)

PfGrx^C88S(SH)₂

roGFP2^C151S(SSG)

2.1 × 10⁴M^–1 s^–1

8.0/25 °C

PfGrx^DM(SSG) ^b

roGFP2^C151S(SH)

PfGrx^DM(SH)

roGFP2^C151S(SSG)

3.5 × 10⁴M^–1 s^–1

8.0/25 °C

PfGrx^C88S(SSG)

roGFP2^C208S(SH)

PfGrx^C88S(SH)₂

roGFP2^C208S(SSG)

9.9 × 10³M^–1 s^–1

8.0/25 °C

PfGrx^DM(SSG) ^b

roGFP2^C208S(SH)

PfGrx^DM(SH)

roGFP2^C208S(SSG)

1.8 × 10⁴M^–1 s^–1

8.0/25 °C

PfGrx^DM(SSG) ^b

GSH

PfGrx^DM(SH)

GSSG

1.2 × 10⁵M^–1 s^{–1 d}

8.0/25 °C

PfGrx^K28A/DM(SSG) ^b

GSH

PfGrx^K26A/DM(SH)

GSSG

5.0 × 10⁴M^–1 s^{–1 d}

8.0/25 °C

PfGrx^D90A/DM(SSG) ^b

GSH

PfGrx^D90A/DM(SH)

GSSG

1.7 × 10⁵M^–1 s^{–1 d}

8.0/25 °C