Fig. 1: Schematic of sequencing technologies used to study astrocytes in stroke. | Nature Communications

Fig. 1: Schematic of sequencing technologies used to study astrocytes in stroke.

From: Integrating single-cell and spatially resolved transcriptomic strategies to survey the astrocyte response to stroke in male mice

Fig. 1

A broad overview of stroke over time (d2 acute, d10 subacute, d21 chronic) was performed using spatially resolved tiles (consisting of multiple cells) with the commercial 10x Visium. Next, astrocyte heterogeneity was examined in suspensions of individual astrocytes isolated from the stroke-injured cortex in the sub-acute stage using 10x Chromium. Finally, to spatially resolve the molecular profiles of individual astrocytes in sub-acute stroke, single astrocytes were captured at various distances from the injury site and analyzed using tDISCO. Biorender was used to create the schematic.

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