Fig. 5: dSTORM imaging of AlexaFluor 647-labeled tubulin in a HeLa cell. | Nature Communications

Fig. 5: dSTORM imaging of AlexaFluor 647-labeled tubulin in a HeLa cell.

From: High-density volumetric super-resolution microscopy

Fig. 5

a Representative frame of SMLFM localization data showing microtubule-stained HeLa cells through 7 perspective views in a hexagonal arrangement (total of 40,000 frames, imaged to completion). Two fiducial markers are indicated by arrows. b The corresponding super-resolved 3D volume, containing 173,314 localizations, color-coded by depth. c Localization rate over the first 2500 frames indicating a mean 3D localization rate of ~ 22 frame−1 (blue line, rolling average over 100 frames) and an upper limit of ~ 40 frame−1, corresponding to a localization density of ~ 0.075 and ~ 0.15 locs μm−2, respectively. d Histogram of detected photons per 3D localization. e Line plots of width of 400 nm illustrate the resolution of individual microtubules as indicated by the triangle and diamond in b.

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