Fig. 6: RTF2 recruits RNase H2 to the replication fork to facilitate removal of genomic ribonucleotides.
From: RTF2 controls replication repriming and ribonucleotide excision at the replisome
a Schematic of neutral comet assay post RNase HII-digestion. b Left: Representative images of neutral comet assay post RNase HII-digestion with olive tail moment. Right: Quantification of olive tail moment in p53-/- MEFs (72 hr after Cre), combined from 4 biological replicates. RNASEH2A KO HeLa cells serve as positive control. c Quantification of olive tail moment in p53-/- MEFs (72 hr after Cre) with or without exogenous RNase HII digestion, combined from 2 biological replicates. d Representative immunoblot of whole cell lysates in primary MEFs 72 hr after Cre showing poly-ADP-ribosylation. e Average ratios from three biological replicates as in (d). f Representative immunofluorescent images of γH2AX staining in primary MEFs 72 hr after Cre. g Quantification of representative experiment of mean nuclear signal of γH2AX from (f). Experiments were conducted at least three times in biological replicates with consistent results for (b), (d), (e), (f), (g). Experiment was conducted twice in biological replicates for (c). Each dot represents one cell for (b), (c), (g). Mean for each sample shown with red line for (b), (c), (g). Experiments were blinded prior to analysis for (b), (c), (g). Error bars represent standard deviation in (e). Significance evaluated by Kruskal-Wallis ANOVA with a Dunn’s post-test. RH2A = RNASEH2A. Source data are provided as a Source Data file.
