Fig. 4: Circuit motifs in presynaptic Tm9 connectivity.

a Heatmap of the Pearson correlation between Tm9 input neuron counts. Statistical testing was done using a two-sided Student’s t-test, ***p < 0.001, followed by Bonferonni correction for multiple comparisons. b Input counts of Tm9 neurons upon K-means clustering of input connectivity with a cluster number of two. Box plots show the median (center line), upper and lower quartiles (box limits), standard deviation (whiskers), and outliers (points). Data from n = 320 columns. c Tm9 input connectivity projected onto PC1 and PC2 with labels representing clusters from K-means (cluster 1, red; cluster 2, blue). d The location of the analyzed Tm9 neurons in the medulla, colored to represent the K-means clusters. e Heatmap showing the Hamming distances between Tm9 columns ordered based on the dendrogram upon hierarchical clustering considering only the variable inputs (excluding L3, Mi4, CT1). f Input motifs from the clustering approach in (e). Each row of the binary image represents a Tm9 neuron (n = 320), the binary color code indicates the presence or absence of input neurons within each cluster. Seven clusters were chosen based on the silhouette coefficient after clustering with different numbers of neurons (n, given to the left of the plot).