Fig. 1: Attenuation of Pol I activity increases lifespan.

a, b Comparison of tif-1A mRNA (a) and pre-rRNA (b) levels in C. elegans wild-type (N2 Bristol) strain and COP2239 strain harboring an ectopic tif-1A gene (ect. tif-1A). RT-qPCR data are shown relative to N2 and normalized to tgb-1 (tubulin gamma chain) mRNA. Each data point represents an independent biological sample of n = 60 worms. c Lifespan analysis of N2 and COP2239 (ect. tif-1A) strains, n = 140 worms. d, e RT-qPCR analysis of tif-1A mRNA (d) and pre-rRNA (e) levels in nematodes treated either with control (Ctrl) RNAi or tif-1A RNAi. Data are relative to Ctrl RNAi and normalized to tgb-1 mRNA. RNA from 3 independent experiments with n = 60 animals per sample was analyzed. f Lifespan analysis of animals treated with Ctrl RNAi or tif-1A RNAi, n = 140 worms. g, h Relative levels of rpoa-2 mRNA (g) and pre-rRNA (h) in nematodes exposed to control (Ctrl) or rpoa-2 RNAi. Samples from 4 (g) or 3 (h) biological replicates (n = 60 animals per sample) were used. i Lifespan analysis of worms treated with Ctrl RNAi or rpoa-2 RNAi, n = 140 worms. For a, b, d, e, g and h data represent mean values ± S.D. P values were determined using a Student’s unpaired t-test. For lifespan analyses in c, f and i, results representative of 3 independent experiments are shown. Survival was scored daily. P values were calculated using the Mantel-Cox test. ****P < 0.0001, **P < 0.01 and *P < 0.05. All statistical tests used were two-sided, the exact P values and statistical analyses are reported in the Source Data file. C. elegans culturing temperatures and RNAi treatment durations for this and other figures are provided in Supplementary Data 23.