Fig. 6: TAG lipolysis mediates longevity effects of the differential Pol I activity.
From: Reducing the metabolic burden of rRNA synthesis promotes healthy longevity in Caenorhabditis elegans
a, b Survival analysis of wild-type (N2 Bristol strain) nematodes exposed to control (Ctrl), rpoa-2 and atgl-1 RNAi, or simultaneously to rpoa-2 and atgl-1 RNAi (a) or Ctrl, ncl-1 and atgl-1 RNAi, or simultaneously to ncl-1 and atgl-1 RNAi (b). c Survival analysis of N2 and prx-5 (ku517) mutant nematodes exposed to Ctrl or rpoa-2 RNAi. d Survival analysis of wild-type worms exposed to Ctrl, rpoa-2, acs-2 RNAi or double RNAi targeting rpoa-2 and acs-2. Survival was scored daily, and n = 140 worms in all tests. Statistics were calculated using Mantel-Cox test. e Mitochondrial oxygen consumption rate (OCR) measured on AD12 in wild-type nematodes treated with Ctrl, tif-1A or ncl-1 RNAi. OCR was normalized to the number of worms and mean ± S.E.M. of n = 85 animals is shown. Statistical analysis was performed by using unpaired t-test on area under the curve values. ns = not significant, ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05. Representative results of 3 independent experiments are shown in all cases. All statistical tests used were two-sided, the exact P values and statistical analyses are reported in the Source Data file. f Schematic representation of the molecular mechanism connecting curbed Pol I activity with the attenuation of metabolic aging. The image was created with BioRender.com.
