Fig. 2: miR-9 suppresses HSV-1 replication and reactivation in mouse TG or TG neurons. | Nature Communications

Fig. 2: miR-9 suppresses HSV-1 replication and reactivation in mouse TG or TG neurons.

From: Neuronal miR-9 promotes HSV-1 epigenetic silencing and latency by repressing Oct-1 and Onecut family genes

Fig. 2: miR-9 suppresses HSV-1 replication and reactivation in mouse TG or TG neurons.The alternative text for this image may have been generated using AI.

a Neurons isolated from mouse TG were transduced with the indicated lentiviruses and then infected with KOS (MOI = 0.1). At the indicated times, supernatants were collected for virus titration. b Mice were inoculated on the cornea with 2 × 105 pfu/eye. TG were harvested at 7 dpi for viral titration. Each dot represents one TG and the n numbers represent the numbers of TG collected. c The numbers of TG with pfu >200 or ≤200 were shown for each virus. d Diagram of neuronal culture models of HSV-1 latency and reactivation. e Using the model depicted in panel d, after the addition of 0.2 μM TSA, supernatants were collected every two days for assays of infectious virus positivity. n.s., non-significant. a n = 4 (left) or 9 (right) biologically independent samples. Sample sizes for other panels are labeled on the figure. Data were analyzed by one-way ANOVA with Dunnett’s multiple comparisons tests (b), two-way ANOVA with Sidak’s multiple comparisons tests (a), two-sided Fisher’s exact tests (c) or Log-rank (Mantel-Cox) tests (e) and are presented as the mean ± s.d. Source data are provided as a Source Data file.

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