Fig. 5: OC2 binds to HSV-1 DNA to stimulate viral gene transcription. | Nature Communications

Fig. 5: OC2 binds to HSV-1 DNA to stimulate viral gene transcription.

From: Neuronal miR-9 promotes HSV-1 epigenetic silencing and latency by repressing Oct-1 and Onecut family genes

Fig. 5: OC2 binds to HSV-1 DNA to stimulate viral gene transcription.The alternative text for this image may have been generated using AI.

a Schematic of OC2 and its truncated mutants. Amino acid positions are labeled at the top. Expanded below are CUT domain residues with the mutated ones in boxes. b Neuro-2a cells were transfected with 200 ng/ml plasmid for 24 h, then infected with ICP0-null virus for 48 h before virus titration. c Crystal structure of OC1 DBDs complexed with DNA adapted by using the PyMOL software (PDB code 2D5V). The dotted lines represent hydrogen bonds whose distances are labeled. d Neuro-2a cells were transfected with 400 ng/μl plasmid for 40 h, then infected with ICP0-null virus (MOI = 3) for 5 h before ChIP-qPCR analysis of the enrichment of transfected Flag-tagged proteins at the indicated promoters using a Flag antibody. e After ChIP performed as in panel d, DNA samples were sequenced. In gray and blue are coverage plots along the HSV-1 genome (with the terminal repeat sequences deleted) for input and immunoprecipitated DNA samples, respectively. Small vertical sticks represent identified peaks in the immunoprecipitated samples. f ChIP-seq signal around transcription start sites (TSS) for host (upper) or viral (lower) genes. g The top-ranked known (left) or de novo (right) motifs identified in host peaks with the P values displayed. n = 2 (e–g), 3 (d, right panel of b) or 4 (left panel of b) biologically independent samples. Data were analyzed by one-way ANOVA with Dunnett’s multiple comparisons tests (b, d) and are presented as the mean ± s.d. Source data are provided as a Source Data file.

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