Fig. 6: αSAA1/2 enhances αPD-1 efficacy in vivo.

a Orthotopic liver murine models implanted with Hepa1-6-luci⁺ cells were separately treated with PBS combined with 0.5% methylcellulose, αPD-1 (10 mg/kg), αSAA1/2 (5 μg/mice), or αPD-1 (10 mg/kg) combined with αSAA1/2 (5 μg/mice) per three days. n = 6 mice. After 18 days, the mice were sacrificed and necropsied. The whole body and organs of mice were weighed after treatment. The dissected tumor tissues were prepared for histological examination, FACS, and immunofluorescence analysis. b Before sacrifice, luciferase marker expression was detected using IVIS. c Images of tumor tissue samples were taken from the necropsied orthotopic mice model. d, e Histograms showed weight changes in the whole mouse, liver tumor, and liver to mouse ratio in different treatment groups. n = 6 mice. f Tumor infiltration of neutrophils (CD11b⁺Ly6G⁺ cells), PD-L1⁺ neutrophils (PD-L1⁺CD11b⁺Ly6G⁺ cells), CD45⁺CD3⁺CD4⁺ T cells, CD45⁺CD3⁺CD8⁺ T cells, IFNγ⁺CD8⁺ T cells, and TNF⁺CD8⁺ T cells were analyzed by FACS in each group. n = 6 mice. g The confocal microscopy images showed the co-expression of SAA, PD-L1, MPO⁺ neutrophils in each treatment group by immunofluorescence analysis. DAPI: blue, MPO: green, PD-L1: orange, and SAA: red. Statistical data presented in this figure show mean ± SEM. ns indicates P > 0.05, *P < 0.05, **P < 0.01, and ***P < 0.001, by one-way ANOVA (e, f). Source data and exact P values are provided as a Source Data file. Illustrations created with BioRender.com.