Fig. 6: Monocyte activation during incubation of hyperalgesic priming.
From: NAAA-regulated lipid signaling in monocytes controls the induction of hyperalgesic priming in mice
A Schematic illustration of the CyTOF protocol used in these experiments. The optimized Stochastic Neighbor Embedding (opt-SNE) plot identifies six CD45+ cell populations in cardiac blood of vehicle- and IL-6-treated mice (n = 5-7 per group): B, T, and NK cells, Ly6Chigh and Ly6Clow monocytes, and neutrophils. The illustration was partially generated with BioRender.com. B opt-SNE plots depicting density heatmaps of cells expressing CCR2 (top), CD43 (middle), and CX3CR1 (bottom) in mice treated with vehicle (left; n = 7) or IL-6 (right; n = 5). C Boxplots showing quantification of activation markers in Ly6Chigh monocytes. D Pie charts showing distribution of CCR2+ (top) and CD43+ (bottom) Ly6Chigh monocytes in vehicle- (left) and IL-6-treated (right) mice. Numbers indicate percentages of total cell number. E, F Boxplots showing quantification of activation markers in Ly6Clow monocytes (E) and neutrophils (F). Data in C, E, and F are presented in box and whiskers plots showing the median, interquartile range, the minimum and maximum values. They were obtained from 5-7 independent biological replicates and analyzed by multiple two-tailed unpaired t test with Bonferroni’s correction. Adjusted P values versus vehicle are indicated. Source data are provided as a Source Data file.
