Fig. 1: HT-eY2H screening of potential human interactors for Ece1-derived peptides.

a The amino acid sequence of each Ece1 peptide. b The schematic workflow based on the high-throughput enhanced yeast two-hybrid (HT-eY2H) system to conduct interactome analysis between each Ece1 peptide (n = 8) and genes in the human ORFeome (n = 13,761). c The plate dotting images of the initial HT-eY2H screening on three types of selective media involving exposure to the candidalysin peptide (Ece1-III). d The plate dotting images of the 2nd HT-eY2H screening on two types of selective media involving exposure to the candidalysin peptide (Ece1-III). Columns 1–6 of Controls: 1 expresses DB and AD plasmids without any fusion; 2 expresses DB-pRB and AD-E2F1 fusion proteins, forming an interaction, and is CHX-sensitive; 3, 4, and 5 express DB-Fos and AD-Jun, DB-GAL4 and AD, and DB-DP and AD-E2F1, respectively, all of which exhibit positive interactions but are CHX-resistant; 6 expresses DB-DP and AD-E2F1, and is CHX-sensitive. The annotation of 1-12 and A-H facilitates our localization of each protein. The colonies were color-categorized according to growth intensity as very strong (red), strong (orange), medium (yellow), weak (cyan), and very weak (blue). Auto-interactive colonies were marked with black squares. Ctrl, Control. e Numbers of potential human protein targets of each peptide.