Fig. 1: Functionalized GO grids protect SNF2h-nucleosome complexes from the air-water interface. | Nature Communications

Fig. 1: Functionalized GO grids protect SNF2h-nucleosome complexes from the air-water interface.

From: Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking

Fig. 1

a Schematic illustrating functionalized graphene-oxide (GO) grids. GO is layered on top of a commercial Quantifoil holey carbon grid. The GO surface is then functionalized with either single-strand DNA (ssDNA) or thiol-poly(acrylic acid-co-styrene) (TAASTY) co-polymer to attract SNF2h-nucleosome complexes away from the air-water interface. b Representative micrograph out of 7423 micrographs of the SNF2h-nucleosome complex on a ssDNA GO grid. Representative 2D classes of particles picked from a ssDNA GO grid are also shown. c Chemical structure of the TAASTY co-polymer. d Representative micrograph out of 2,020 micrographs of the SNF2h-nucleosome complex on a TAASTY GO grid. Representative 2D classes of particles picked from a TAASTY GO grid are also shown.

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