Fig. 4: Performance of TDN-Ng MNs for cfDNA and RNA in vitro.
a Real-time I response targeting variable concentrations of target cfDNA by the TDN-Ng MN patch in stimulated ISF, 37 °C, pH 7.4, and 0.01 M PBS. b Calibration curve of I response versus target concentration. Data are presented as the mean values ± SDs, n = 3 independent experiments. c Slope values of the curves collected from Fig. 4b calculated by differentiation, using two-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, p value of 0.0000678, 0.000232, 0.000026, 0.0000483 for 0.3, 3, 30, 300 fM groups respectively, data presented as the mean values ± SDs, n = 3 independent experiments. d The long-term stability of the TDN-Ng MN patch in vitro for 20 days, incubated in stimulated tissue at 37 °C, and monitoring 3 × 10−14 M target cfDNA on a chip under reverse iontophoresis (10 V). Data are presented as the mean values ± SDs, n = 3 independent experiments, by polynomial fitting of 4 orders. e Specificity of the TDN-Ng platform for 0.3 nM target and other interfering nucleic acids using 50 mM [Fe(CN)6]3–/4–, using two-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, p value of 0.00001, 0.000048, 0.000173 referring to, without NgAgo for EBV, gDNA 3 for sepsis, gDNA 3 for organ groups respectively, data presented as the mean values ± SDs, n = 3 independent experiments. f The anti-interference performance of the TDN-Ng MN patch under different concentrations of fetal bovine serum for 3 × 10−12 M target cfDNA. g Investigation of the binding ability of the NgAgo-gDNA complex with 3 nM RNA-168 and RNA-351, incubated at 37 °C for 60 min, analysed by two-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, p value of 0.0083, 0.0071, for RNA-168, RNA-351 groups respectively, data presented as the mean values ± SDs, n = 3 independent experiments, repeated time = 3. h Dynamic change in RNA-168 and RNA-351 recording by a TDN-Ng MN; steps 1, 2, and 3 refer to real-time monitoring of the 3 × 10−14 M target during 0–30 min, the 3 × 10−13 M target during 31–63 min, and the 3 × 10−12 M target during 64–96 min, respectively.
