Fig. 1: Genome-wide translated ORFs identified in five eukaryotic species by analyzing ribosome profiling datasets. | Nature Communications

Fig. 1: Genome-wide translated ORFs identified in five eukaryotic species by analyzing ribosome profiling datasets.

From: Widespread stable noncanonical peptides identified by integrated analyses of ribosome profiling and ORF features

Fig. 1

a Steps of ribosome profiling data analyses. b The five eukaryotic species included in the analyses and their phylogenetic relationship. c The ribosomal A-site adjusted reads used to identify genome-wide translated ORFs in humans. d–f The read distribution features used to distinguish translated ORFs vs. other candidate ORFs: the fraction of in-frame reads (d), the relative fraction of codons supporting in-frame translation (e), and PME measuring uniformity of read distribution (f). The boxes are bounded by the 25 and 75 percentiles and the center represents the median. The whiskers extend from each edge of the box to indicate the 1.5x interquartile range. We randomly sampled 1000 ORFs in each group for comparison. The two-sided Wilcoxon Rank Sum Test P-values comparing the translated ORFs vs. other candidate ORFs are shown. g The statistics of translated ORFs identified across species, grouped by transcript type and ORF location. h The fraction of protein-coding genes with noncanonical translation.

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