Fig. 7: Excretory canal defects in the nematode C. elegans with point mutation F38D in the exc-4 gene.
From: Chloride intracellular channel (CLIC) proteins function as fusogens
a Logo plot of the fractional abundance of residues flanking CLIC5-F34D among human CLIC1-6 and C. elegans EXC-4. b Closeup view of CLIC5 F34 (left) and EXC-4 F38 (right), colored according to Consurf conservation score analyses. c Representative images of L4 stage worms expressing an excretory canal fluorescent marker (green). Control worm (upper panel) with an excretory canal extending from the pharynx to the tail, and exc-4 mutant worm (lower panel) with an excretory canal extending only to the vulva and harboring visible cysts along the pharyngeal region are shown. Yellow arrows indicate the end of the canal in each worm. Magnified pharyngeal regions are presented on the right. Bars = 50 μm (left panels) and 20 μm (right panels). d Outgrowth scores of control vs. exc-4 mutant worms. Significance was calculated with the two-sided Mann-Whitney U test, ****P < 0.0001. e Percentage of worms with cysts along the excretory canal in control and exc-4 mutant worms.
