Fig. 1: Centrosome amplification in the primary CD4+ T cells purified from people living with HIV-1. | Nature Communications

Fig. 1: Centrosome amplification in the primary CD4+ T cells purified from people living with HIV-1.

From: Centrosome amplification and aneuploidy driven by the HIV-1-induced Vpr•VprBP•Plk4 complex in CD4+ T cells

Fig. 1: Centrosome amplification in the primary CD4+ T cells purified from people living with HIV-1.

a Representative three-dimensional structured illumination microscopy (3D-SIM) images showing over-duplicated centrosomes (marked by Cep152 and γ-tubulin signals) in primary CD4+ T cells purified from healthy individuals and people living with HIV-1. Purified cells were immunostained immediately without culturing them. Boxes, area of enlargement. b Data showing the percentage of CD4+ T cells that exhibit multiple centrosomes. Quantification was performed with the cells purified from the blood of 14 healthy people [a total of n = 14 biologically independent samples examined over two independent experiments (1st set: samples 1–2 and 2nd set: samples 3–14 in Supplementary Fig. 1c)] and 10 people living with HIV-1 [a total of n = 10 biologically independent samples examined over two independent experiments (1st set: samples 1–5 and 2nd set: samples 6–10 in Supplementary Fig. 1d, e)]. c Quantified data showing the reduction of primary CD4+ T cells with multiple centrosomes after ART. The data were obtained by analyzing four paired samples (a total of n = 8 biologically independent samples) obtained before and after ART from the same people with HIV-1 (the #1–4 individuals listed in Supplementary Fig. 1d) Bars, mean ± s.d.; P values, unpaired two-tailed t-tests. Detailed clinical data for all the samples analyzed here are provided in Supplementary Fig. 1c–e.

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