Fig. 2: Direct interaction between VprBP AR and Plk4 CPB. | Nature Communications

Fig. 2: Direct interaction between VprBP AR and Plk4 CPB.

From: Centrosome amplification and aneuploidy driven by the HIV-1-induced Vpr•VprBP•Plk4 complex in CD4+ T cells

Fig. 2: Direct interaction between VprBP AR and Plk4 CPB.

a Two independent mass spectrometry analyses showing that VprBP and DDB1 co-purified with the indicated Plk4 ligands. The peptide counts of the listed proteins co-purified with the ligands are shown. A ZZ-tagged Plk4 CPB (581–884) and a FLAG-tagged Plk4 CTD (581–970) were used as ligands for Expt. 1 and 2, respectively (see Methods for details). b–d Coimmunoprecipitation analyses performed with HEK293T cells transfected with the indicated constructs. Residue numbers are shown in the schematic diagram. KD kinase domain, CPB cryptic polo-box domain, PB3 polo-box 3: NTD, N-terminal domain, CTD C-terminal domain, WD40 WD40 domain, AR Acidic region. Numbers in the immunoblots represent relative signal intensities. e SEC profile and SDS-PAGE showing coeluting MBP-VprBP(1446–1507) and CPB(581–808) in the Coomassie Brilliant Blue-stained gel. Asterisk, contaminating protein.

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