Fig. 8: Correlation of the FC and IRF localization on 150 nm sections by CLEM. | Nature Communications

Fig. 8: Correlation of the FC and IRF localization on 150 nm sections by CLEM.

From: In-section Click-iT detection and super-resolution CLEM analysis of nucleolar ultrastructure and replication in plants

Fig. 8

Imaging of 150 nm sections on TEM post-contrasted with uranyl acetate and lead citrate on 150 nm sections in the fas1 mutants (TEM images in A–C) with visible nucleus (N), nucleolus (No) and fibrillar centres (FC), marked with a white arrow. Fluorescence imaging of replicating DNA without (D) and with intranucleolar replication foci (IRFs—E, F). The overlay images (G–I) and corresponding line profiles (J–L) show the level of colocalization between IRFs and FCs. Experiments were performed in two biological and two technical replicates. ROI region of interest, px pixels, TEM transmission electron microscopy, SD spinning disk. Scale bar: 2 µm.

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