Fig. 2: DNA nanotube colocalization and bundling by starPEG-(KA7)4.
From: Triggered contraction of self-assembled micron-scale DNA nanotube rings
a Schematic illustration of the DNA nanotube colocalization assay with a biotinylated DNA nanotube A (green) bound to biotinylated bovine serum albumin-coated glass slides upon addition of 0.2 wt% neutravidin. StarPEG-(KA7)4 crosslinks DNA nanotube B (yellow) to the immobilized nanotube A. b Composite confocal image of the immobilized DNA nanotube A (green, 6-FAM, λex = 488 nm) and DNA nanotube B (yellow, Atto633-labeled, λex = 640 nm) illustrating their colocalization. Scale bar: 10 μm. c Confocal images of DNA nanotubes (30 nM DNA tiles, yellow, Atto633-labeled, λex = 640 nm) and 200 nM starPEG-(KA7)4 (cyan, TAMRA-labeled, λex = 561 nm). Scale bar: 10 μm. d Colocalization intensity of starPEG-(KA7)4 (TAMRA-labeled, λex = 561 nm) with DNA nanotubes (30 nM DNA tiles) (Atto633-labeled, λex = 640 nm, Mean ± SD, n = 10 DNA nanotube bundles analyzed per condition, exponential fit plotted as red line, \(y={y}_{0}+(P-{y}_{0})(1-\exp (-kx)),\,{y}_{0}=0.85,\,P=53.49,\,k=0.018\)). e TEM micrographs of DNA nanotubes (30 nM DNA tiles) in the absence of starPEG-(KA7)4 (left) and in presence of 200 nM starPEG-(KA7)4 (right). Scale bar: 200 nm. f DNA nanotube bundle thickness over starPEG-(KA7)4 concentration (Mean ± SD, n = 100 DNA nanotube bundles analyzed per condition, 30 nM DNA tiles, exponential fit plotted as red line, \(y={y}_{0}+(P-{y}_{0})(1-\exp (-kx)),\,{y}_{0}=11.79,P=138.80,\,k=0.017\)). Source data are provided as a Source Data file.
