Fig. 1: Identifying a biosensor responsive to amaryllidaceae alkaloid intermediates.
From: Biosensor and machine learning-aided engineering of an amaryllidaceae enzyme
a. Abbreviated biosynthetic plant pathways for therapeutic amaryllidaceae alkaloids. b Response of the RamR transcription factor to amaryllidaceae alkaloid pathway intermediates norbelladine and 4′-O-methylnorbelladine. Error bars represent the S.D. +/− the mean. Experiments were conducted in biological triplicate. c Structure of RamR (PDB: 3VVX_A) docked with 4′-O-methylnorbelladine using GNINA (see “Methods”). Predicted ligand-interacting residues are highlighted green.
