Fig. 3: Monitoring Nb4OMT activity with the 4NB2 biosensor.
From: Biosensor and machine learning-aided engineering of an amaryllidaceae enzyme
a Schematic representation of the biosensor-monitored enzymatic reaction within E. coli cells. Blue and purple hexagons denote norbelladine and 4′-O-methylnorbelladine, respectively. The schematic was designed using a vector graphics editor. b Correlation between cell population fluorescence and biosynthesized 4′-O-methylnorbelladine, measured by high-performance liquid chromatography. All data was performed in biological triplicate. Error bars represent S.D. +/− the mean. c Chromatographic traces of supernatant collected from E. coli cells expressing an active Nb4OMT enzyme (green) or an empty plasmid (red). Traces for norbelladine and 4′-O-methylnorbelladine standards are shown in blue and purple, respectively.
