Table 1 Kinetic and thermal parameters of the wild-type and mutant Nb4OMTs
From: Biosensor and machine learning-aided engineering of an amaryllidaceae enzyme
Enzyme | kcat/Km (μM−1 min−1) | kcat (min−1) | Km (μM) | Tm (oC) |
|---|---|---|---|---|
Wild-type Nb4OMT | 1.18 (0.85–1.69) | 73 (63–89) | 62 (37–104) | 52.8 (52.8–52.8) |
A53M mutant | >2.1 | >190 | <90 | 54.5 (54.3–54.6) |
E36P/G40E/A53M mutant | >1.4 | >120 | <83 | 58.4 (58.4–58.4) |
