Fig. 1: The insertion of an ERVK[2-1-LTR] element in the APOB gene causes cholesterol deficiency in Holstein-Friesian cattle. | Nature Communications

Fig. 1: The insertion of an ERVK[2-1-LTR] element in the APOB gene causes cholesterol deficiency in Holstein-Friesian cattle.

From: GWAS reveals determinants of mobilization rate and dynamics of an active endogenous retrovirus of cattle

Fig. 1

a Genomic structure of the bovine APOB gene (chr11: 77,885,988-77,927,967 bp)(upper lane), and liver cDNA sequence coverage tracks for a homozygous wild-type animal (+/+, grey) and an affected homozygous mutant animal (MUT/MUT, red), highlighting the transcriptional shutdown in exon 5, with partial retention of intron 4 (see also Supplementary Fig. 1). b Schematic representation of the ~ 6.8 Kb ERVK[2-1-LTR] element sense insertion with the translation of its full-length sequence in the three frames (+ 1, + 2, + 3), revealing the absence of intact open reading frames (ORFs) in the proviral sequence flanked by two identical long terminal repeats (LTRs). Stop codons are represented by black vertical bars. Partial GAG and ENV ORFs are shown as grey rectangles (frame +2).

Back to article page