Fig. 5: Transcriptomic profiling of hematopoietic related cells in the control and KO AGM region by scRNA-Seq.

a UMAP plots visualized nine clusters from hematopoietic related cells (EC, HC and Mk). b Bar graph showing the portions of each sub-cluster (EC and HC) in the control and KO AGM regions. c The schematic diagram for detecting the diameter of aorta in the detected sections based on the staining of CD34. Red = CD34. d The diameters and areas of aorta in the control and KO group. Error bars represent mean ± SEM. n = 15 sections from 3 independent embryos, **p < 0.01. Circles = control (Ctr), inverted squares = KO. e Bar charts showing the percentage contribution of different cell cycle phases in the control and KO cells. f Flow cytometric analysis confirmed the cell cycle status of pre-HSC II in the E11.5 AGM region. Error bars represent mean ± SEM. n = 4 biologically independent experiments, **p = 0.0047. g UMAP plots visualized the ten clusters from vEC, aEC, HEC and pre-HSCs. h Violin graphs displaying the key featured genes in each cell cluster. i Comparison analysis showing cell proportions of distinct clusters from EC and pre-HSCs between KO and control group. Statistical significance was determined by one side Student’s t-test.